SPP 1530: Flowering Time Control - from Natural Variation to Crop Improvement

PP-24: Quint, Große, Weisshaar

Evolutionary transcriptomics of floral transition

This interdisciplinary project takes an evolutionary transcriptomics approach on the transition from the vegetative to the reproductive phase in flowering plants. In collaboration with four partners within SPP 1530, we will generate high-resolution time series of transcriptomes based on next generation sequencing of RNA populations before, during, and after floral transition in Arabidopsis thaliana, Capsella rubella, Brassica napus, Beta vulgaris, Vitis vinifera, and Hordeum vulgare. Based on these data, we aim (i) at identifying novel potential regulators of floral transition by comparative transcriptomics and (ii) at uncovering the possible presence of an evolutionarily conserved phylotranscriptomic pattern of floral transition. By the comparative transcriptomics approach we will construct gene regulatory networks (i) to understand the known floral transition network on an evolutionary level and (ii) to identify novel potential regulators of floral transition, which we will functionally evaluate by reverse genetics. By the phylotranscriptomics approach, which combines phylogenetic and whole-transcriptome information, we will assess whether specific ontogenetic stages before, during, or after floral transition are evolutionarily conserved or, alternatively, evolutionarily variable; the latter pointing to adaptational potential. The combination of both approaches will allow us to view developmental phase change from an evolutionary perspective. Furthermore, we will develop a graphical web-based user interface to make phylotranscriptomics available and applicable to all members of the SPP 1530 and other research groups worldwide.


Project-related publications:

Drost HG, Janitza P, Grosse I, Quint M (2017) Cross-kingdom comparison of the developmental hourglass. Curr Opin Genet Dev. 2017 Mar 24;45:69-75. http://www.sciencedirect.com/science/article/pii/S0959437X16302040

Trenner J, Poeschl Y, Grau J, Gogol-Döring A, Quint M, Delker C (2017). Auxin-induced expression divergence between Arabidopsis species may originate within the TIR1/AFB/AUX/IAA-ARF module. J Exp Bot. 2017 Jan 1;68(3):539-552. https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erw457

Drost HG,  Bellstädt J, Ó'Maoiléidigh DS, Silva AT, Gabel A, Weinholdt C, Ryan PT, Dekkers BJ, Bentsink L, Hilhorst HW, Ligterink W, Wellmer F, Grosse I, Quint M (2016). Post-embryonic Hourglass Patterns Mark Ontogenetic Transitions in Plant Development. Mol Biol Evol. 2016 May;33(5):1158-63. https://academic.oup.com/mbe/article-lookup/doi/10.1093/molbev/msw039

Raschke A, Ibañez C, Ullrich KK, Anwer MU, Becker S, Glöckner A, Trenner J, Denk K, Saal B, Sun X, Ni M, Davis SJ, Delker C, Quint M.(2015). Natural variants of ELF3 affect thermomorphogenesis by transcriptionally modulating PIF4-dependent auxin response genes.BMC Plant Biol. 2015 Aug 14;15:197. https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0566-6

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