SPP 1530: Flowering Time Control - from Natural Variation to Crop Improvement

PP-22: Jung

Unravelling a genetic network for bolting time regulation in Beta species to breed winter sugar beet

Sugar beet is a biennial species bolting after exposure to extended cold. Therefore, field production with winter grown beets is impossible. However, winter beets could be an interesting alternative for beet production due to their higher yield potential. We have cloned two key regulators for bolting time control in beet, BTC1 and BvBBX19. We assume that both genes jointly regulate the expression of downstream targets such as BvFT1 and BvFT2. We will study the function of both genes in beet and after transformation into Arabidopsis thaliana in collaboration with two partners from the priority program. Transgenic beets overexpressing BTC1 start bolting after winter like common sugar beet. However, transgenic beets with >1 BTC1 copy generally failed to bolt probably due to BTC1 cosuppression. These results provided an incentive to breed winter beets. We will produce hybrids with low or absent BTC1 expression after crossing two BTC1 overexpression lines. Hybrid beets will be grown over winter in the climate chamber, in a gauzehouse (semi field conditions) and in the field. In a second approach we will produce double recessive beets (btc1 and Bvbbx19) with severely delayed bolting after winter. Those beets could be grown worldwide without legal restrictions because they are non-transgenic. Moreover, we will collaborate in a ‘phylotranscriptomics’ experiment with another partner in the frame of PP1530 to grow sugar beet under standardized conditions in the climate chamber.



Project-related publications

Dally N, Eckel M, Batschauer A, Höft N, Jung C (2018). Two CONSTANS-LIKE genes jointly control flowering time in beet. Scientific Reports-UK 8: 16120 https://www.nature.com/articles/s41598-018-34328-4

Höft N, Dally N, Jung C (2018). Sequence variation in the bolting time regulator BTC1 changes the life cycle regime in sugar beet. Plant Breeding 2018;00:1–11. https://doi.org/10.1111/pbr.12579

Höft N, Dally N, Hasler M, Jung C (2018). Haplotype variation of flowering time genes of sugar beet and its wild relatives and the impact on life cycle regimes. Frontiers in Plant Science, 8:2211. https://www.frontiersin.org/articles/10.3389/fpls.2017.02211/full

Jarvis DE, Ho YS, Lightfoot DJ, Schmöckel SM, Li B, Borm TJA, Ohyanagi H, Mineta K, Michell CT, Saber N, Kharbatia NM, Rupper RR, Sharp AR, Dally N, Boughton BA, Woo YH, Gao G, Schijlen EGWM, Guo X, Momin AA, Negrão S, Al-Babili S, Gehring C, Roessner U, Jung C, Murphy K, Arold ST, Gojobori T, Linden CGvd, van Loo EN, Jellen EN, Maughan PJ, Tester M (2017). The genome of Chenopodium quinoa. Nature 542: 307-312 http://www.nature.com/nature/journal/v542/n7641/full/nature21370.html

Tränkner C, Lemnian IM, Emrani N, Pfeiffer N, Tiwari SP, Kopisch-Obuch FJ, Vogt SH, Müller AE, Schilhabel M, Jung C, Grosse I (2016). A Detailed Analysis of the BR1 Locus Suggests a New Mechanism for Bolting after Winter in Sugar Beet (Beta vulgaris L.). Frontiers in Plant Science 7: 1662 https://doi.org/10.3389/fpls.2016.01662

Blümel M, Dally N and Jung C (2015). Flowering time regulation in crops - what did we learn from Arabidopsis? Current Opinion in Biotechnology, 32: 121-129, doi:10.1016/j.copbio.2014.11.023

Dally N, Xiao, K, Holtgräwe D, Jung C (2014). The B2 flowering time locus of beet encodes a zinc finger transcription factor. PNAS USA 111 (28): 10365–10370, doi: 10.1073/pnas.1404829111

Pfeiffer N, Tränkner C, Lemnian I, Grosse I, Müller AE, Jung C, Kopisch-Obuch FJ (2014). Genetic analysis of bolting after winter in sugar beet (Beta vulgaris L.). Theoretical Applied Genetics 127:2479-2489, doi:10.1007/s00122-014-2392-x

Vogt S, Weyens G, Lefèbvre M, Bork B, Schechert A, Müller AE (2014). The FLC-like gene BvFL1 is not a major regulator of vernalization response in bennial beets. Frontiers in Plant Science (5), doi: 10.3389/fpls.2014.00146

Melzer S, Müller AE, Jung C (2014). Genetics and genomics of flowering time regulation in sugar beet In: Genomics of Plant Genetic Resources, Vol. 2., Crop productivity, food security and nutritional quality. (Eds: R. Tuberosa, A. Graner, E. Frison). Springer 2014. ISBN: 978-94-007-7575-6, S.3-27.

Frerichmann SLM, Kirchhoff M, Müller AE, Scheidig AJ, Jung C, Kopisch-Obuch FJ (2013). EcoTILLING in Beta vulgaris reveals polymorphisms in the FLC-like gene BvFL1 that are associated with annuality and winter hardiness. BMC Plant Biology 13:52, doi:10.1186/1471-2229-13-52

Pin P, Zhang W, Vogt S, Dally N, Büttner B, Schulze-Buxloh G, Jelly NS,Chia TYP, Mutasa-Göttgens E, Dohm JC, Himmelbauer H, Weisshaar B,Kraus J,Gielen JJL, Lommel M, Weyens G, Wahl B, Schechert A, Nilsson O, Jung C, Kraft T, Müller AE (2012). The Role of a Pseudo-Response Regulator Gene in Lifecycle Adaptation and Domestication of Beet Current Biology 22 (12): 1095-1101, doi:http://dx.doi.org/10.1016/j.cub.2012.04.007


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