SPP 1530: Flowering Time Control - from Natural Variation to Crop Improvement

PP-14: Staiger

Unravelling the role of a novel autonomous pathway component in FTi control by small RNA profiling and cross-species comparison

We will compare the role of an RNA-binding protein in floral transition in Arabidopsis thaliana and Hordeum vulgare. The RNA-binding protein AtGRP7 promotes floral transition mainly by downregulating the floral repressor FLC via the autonomous pathway. We will globally compare the small RNA component of the transcriptome during FTi regulation in wild type plants and AtGRP7 overexpressors by deep sequencing. This will extend the knowledge on small RNAs associated with floral transition and provide insights into the regulatory network downstream of this RNA-binding protein. Further, we will address the question how AtGRP7 orthologues function in crop species lacking FLC homologues. A barley line with highly elevated levels of the AtGRP7 orthologue HvGR-RBP1 shows accelerated FTi and preanthesis development when compared to a near-isogenic parent with very low expression of this gene. We will characterize in detail flowering of this line with respect to different photoperiods and its vernalization requirement. We will employ a TILLING approach to further delineate the function of HvGR-RBP1 in flowering. A candidate gene approach to identify downstream targets will provide insights into the signaling pathways through which HvGR-RBP1 influences FTi. This project contributes to the development of a functional cross-species network of FTi regulators, the major strategic aim of the SPP.


Project-related publications:


Köster T, Meyer K, Weinholdt C, Smith LM, Lummer M, Speth C, Grosse I, Weigel D, Staiger D (2014). Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis. Nucl. Acids Res., doi: 10.1093/nar/gku716

Johansson M & Staiger D (2014).  SRR1 is essential to repress flowering in noninductive conditions in Arabidopsis thaliana. J. Exp. Bot., doi: 10.1093/jxb/eru317

Steffen A, Fischer AM, Staiger D (2014) Determination of photoperiodic flowering time control in Arabidopsis and barley. In “Plant Circadian Networks” Methods in Molecuar Biology, in press

Löhr B, Streitner C, Steffen A, Lange T, Staiger D (2014). A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis. Mol. Biol. Rep. doi:10.1007/s11033-013-2878-7.

Streitner C, Simpson CG, Shaw P, Danisman S, Brown JW, Staiger D (2013). Small changes in ambient temperature affect alternative splicing in Arabidopsis thaliana. Plant Signal Behav 8 (7): e24638, doi: 10.4161/psb.24638

Staiger D, Shin J, Johansson M, Davis SJ (2013). The circadian clock goes genomic. Genome Biology 2013, 14:208, doi:10.1186/gb-2013-14-6-208

Staiger D, Brown JWS (2013). Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses. Plant Cell 25, http:/​/​dx.​doi.​org/​10.​1105/​tpc.​113.​113803

Schmal C, Reimann P, Staiger D (2013). A Circadian Clock-Regulated Toggle Switch Explains AtGRP7 and AtGRP8 Oscillations in Arabidopsis thaliana. PLoS Computational Biology 9, e1002986.

Baerenfaller K, Bastow R, Beynon J, Brady S, Brendel V, Donaldson S, Dooley R, Forster M, Friesner J, Gifford D, Grotewold E, Gutierrez R, Huala E, Jaiswal P, Joshi H, Kersey P, Liu L, Loraine A, Lyons E, May S, Mayer K, MacLean D, Meyers B, Mueller L, Muller R, Muller HM, Ouellette F, Pires JC, Provart N, Staiger D., Stanzione D, Taylor J, Taylor C, Town C, Toyoda T Vaughn M, Walsh S, Ware D, Weckwerth W (2012) Taking the Next Step: Building an Arabidopsis Information Portal. Plant Cell 24, 2248-2256.

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