SPP 1530: Flowering Time Control - from Natural Variation to Crop Improvement

PP-8: Jung

Genomic dissection of floral transition in Brassica napus towards crop improvement by life cycle adaptation and hybrid yield increase

Jung Rapeseed_newRapeseed (Brassica napus L.) is the most important oil crop of the temperate zone with its largest cultivation areas in Europe, North America and Australia. Due to important breeding progress in the past 40 years, its oil is used for human consumption and its meal became an important livestock feed. However, the genetic basis of commonly used rapeseed is still quite narrow which is limiting the breeding progress for this species.

Floral induction is a key developmental switch in seed production. Thus, identification of the genetic factors that integrate the developmental and environmental signals that promote or inhibit flowering are key for sustainable agriculture under distinct environmental conditions.

This project has three major aims:

  • a comprehensive survey of flowering time genes in B. napus to detect yet unknown flowering time (FTi) regulators by transcriptome analysis, promotor analysis, linkage mapping and association mapping of FTi orthologs

  • the detection and phenotypic evaluation of new FTi mutants and sequence variants by TILLING and EcoTILLING

  • Analysis of the genome-wide transcriptional variation in three different B. napus phenological ecotypes (winter and spring types) at Flowering transition stage

The development of novel high-throughput sequencing methods (NGS) has provided a new tool for genome and transcriptome studies (RNA-seq) and will facilitate gene expression analysis of the candidate genes. Furthermore, a TILLING protocol was established for B. napus which now allows routine selection of mutants.

Project-related publications:

Guo Y, Harloff HJ, Jung C, Molina C (2014). Mutations in single FT- and TFL1-paralogs of rapeseed (Brassica napus L.) and their impact on flowering time and yield components. Frontiers in Plant Science 5:282, doi: 10.3389/fpls.2014.00282

Zou X, Suppanz I, Raman H, Hou J, Wang J, Long Y, Jung C, Meng J (2012). Comparative analysis of FLC homologues in Brassicaceae provides insight into their role in the evolution of oilseed rape. PLoS One 7 (9): e45751, doi: 10.1371/journal.pone.0045751

Wang N, Qian W, Suppanz I, Wei L, Mao B, Long Y, Meng J, Müller AE, Jung C (2011). Flowering time variation in oilseed rape (Brassica napus L.) is
associated with allelic variation in the FRIGIDA homologue Bna.FRO.a. J. Exp. Bot. 62(15):5641-5658

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