Development of an early flowering system for poplar breeding and biosafety research
This project aims to generate a more efficient and reliable flowering in poplar. Early flowering would be very valuable to forest tree breeding and biosafety research on genetic containment. Several approaches will be tested using gene stacking of FTi and flower meristem/organ identity genes. Early flowering and growth performance of the different transgenic lines, as well as gene expression of selected poplar FTi and meristem/organ identity genes will be evaluated.
Pakull B, Kersten B, Lueneburg J, Fladung M (2014). A simple PCR-based marker to determine sex in aspen. Plant Biol (Stuttg), in press.
Ahuja MR, Fladung M (2014). Integration and inheritance of transgenes in crop plants and trees. Tree Genetics & Genomes, in press.
Kersten B, Pakull B, Groppe K, Lueneburg J, Fladung M (2014). The sex-linked region in Populus tremuloides Turesson 141 corresponds to a pericentromeric region of about two million base pairs on P. trichocarpa chromosome 19. Plant Biol (Stuttg) 16(2):411-41
Fladung M, Hoenicka H, Raj Ahuja M (2013). Genomic stability and long-term transgene expression in poplar. Transgenic Res 22(6):1167-78
Schroeder H, Hoeltken AM, Fladung M (2012). Differentiation of Populus species using chloroplast single nucleotide polymorphism (SNP) markers - essential for comprehensible and reliable poplar breeding. Plant Biol. 14: 374-381.
Hoenicka H, Lautner S, Klingberg A, Koch G, El-Sherif F, Lehnhardt D, Zhang B, Burgert I, Odermatt J, Melzer S, Fromm J, Fladung M (2012). Influence of over-expression of the Flowering Promoting Factor 1 gene (FPF1) from Arabidopsis on wood formation in hybrid poplar (Populus tremula L. x Populus tremuloides Michx.). Planta 235: 359-373
Fladung M, Polak O (2012). Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery. BMC Genomics 13:61
Fladung M, Altosaar I, Bartsch D, Baucher M, Boscaleri, F, Gallardo F, Haggman H, Hoenicka H, Nielsen K, Paffetti D, Seguin A, Stotzky G, Vettori C (2012). European discussion forum on transgenic tree biosafety. Nature Biotechnology 30 (1):37-38