SPP 1530: Flowering Time Control - from Natural Variation to Crop Improvement

PP-3: Davis

Directing floral timing through genetic variation in the plant circadian clock

Flowering time is strongly regulated by the circadian clock, which drives photoperiodic flowering. We recently explored natural allelic diversity of the clock in the dicot Arabidopsis and found a "memory" of the proceeding environment. Furthermore, we showed that clock variation has a large role in directing flowering time under field conditions. Cloning of one circadian quantitative trait locus revealed variation at the flowering-time gene EARLY FLOWERING 3 (ELF3). Here we will further explore allelic variation in clock genes to define key loci that direct photoperiodic flowering. Firstly, we will complete the construction of new Arabidopsis recombinant inbred populations derived from accessions originating from extremely differing latitudes, and map the genomes of these lines at kilobase resolution. These populations will be scored for variation in the clock and flowering time; dynamic correlations will be constructed. Together, components underling clock-gene variation that directs seasonal flowering will be identified. Secondly, we will examine the molecular genetics of circadian control of flowering in the monocot barley using existing and newly generated variation at barley ELF3. This gene is the likely direct regulator of the seasonality locus Ppd-H1. This second program should reveal dicot/monocot clock conservations and identify allelic variation at the circadian-clock gene ELF3 that could be directly used in barley breeding programs.


Project-related Publications:

Mueller LM, von Korff M, Davis SJ (2014). Connections between circadian clocks and carbon metabolism reveal species-specific effects on growth control. J Exp Bot, doi: 10.1093/jxb/eru117

Sarnowska EA, Rolicka AT, Bucior E, Cwiek P, Tohge T, Fernie AR, Jikumaru Y, Kamiya Y, Franzen R, Schmelzer E, Porri A, Sacharowski S, Gratkowska DM, Zugaj DL, Taff A, Zalewska A, Archacki R, Davis SJ, Coupland G, Koncz C, Jerzmanowski A, Sarnowski TJ. (2013). DELLA-Interacting SWI3C Core Subunit of Switch/Sucrose Nonfermenting Chromatin Remodeling Complex Modulates Gibberellin Responses and Hormonal Cross Talk in Arabidopsis. Plant Physiol.163(1):305-17. doi: 10.1104/pp.113.223933.

Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nesi AN, Stitt M, Fernie AR, Davis SJ (2013). TIME FOR COFFEE is an Essential Component in the Maintenance of Arabidopsis thaliana Metabolic Homeostasis. Plant J. doi: 10.1111/tpj.12292. [Epub ahead of print]

Staiger D, Shin J, Johansson M, Davis SJ (2013). The circadian clock goes genomic. Genome Biology 2013, 14:208, doi:10.1186/gb-2013-14-6-208

Anwer MU and Davis SJ (2013). An overview of natural variation studies in the Arabidopsis thaliana circadian clock. Semin. Cell Dev. Biol. 24, 422-429, doi: http://dx.doi.org/10.1016/j.semcdb.2013.03.006.

Campoli C, Pankin A, Drosse B, Casao CM, Davis SJ, and von Korff M (2013): HvLUX1 is a candidate gene underlying the early maturity 10 locus in barley: phylogeny, diversity, and interactions with the circadian clock and photoperiodic pathways. New Phytol., n/a-n/a, doi: 10.1111/nph.12346.

Shin J, Du S, Bujdoso N, Hu Y, and Davis S (2013). Overexpression and loss-of-function at TIME FOR COFFEE results in similar phenotypes in diverse growth and physiological responses. J. Plant Biol. 56, 152-159, doi: 10.1007/s12374-013-0091-9.

Shin J,  Anwer MU, and Davis SJ (2013). Phytochrome-Interacting Factors (PIFs) as Bridges between Environmental Signals and the Circadian Clock: Diurnal Regulation of Growth and Development. Mol. Plant 6, 592-5, doi: 10.1093/mp/sst060.

Budjoso N & Davis SJ (2013). Mathematical modeling of an oscillating gene circuit to unravel the circadian clock network of Arabidopsis thaliana. Front Plant Sci 4:3, doi: 10.3389/fpls.2013.00003

Archacki R, Buszewicz D, Sarnowski TJ, Sarnowska E, Rolicka AT, Tohge T, Fernie AR, Jikumaru Y, Kotlinski M, Iwanicka-Nowicka R, Kalisiak K, Patryn J, Halibart-Puzio J, Kamiya Y, Davis SJ, Koblowska MK, Jerzmanowski A (2013). BRAHMA ATPase of the SWI/SNF chromatin remodeling complex acts as a positive regulator of gibberellin-mediated responses in Arabidopsis. PLoS One 8(3): e58588, doi: 10.1371/journal.pone.0058588

Herrero E, Kolmos E, Budjoso N, Yuan Y, Uhlworm H, Coupland G, Saini R, Jaskolski M, Webb A, Goncalves J, Davis SJ (2012). EARLY FLOWERING4 recruitment of EARLY FLOWERING3 in the nucleus sustains the Arabidopsis circadian clock.  The Plant Cell 24:428-443

Faure S, Turner A, Gruszka D, Christodoulou V, Davis SJ, von Korff M, Laurie DA (2012). Mutation at the circadian clock gene EARLY MATURITY8 adapts domesticated barley (Hordeum vulgare) to short growing seasons. Proc. Natl. Acad. Sci. USA 109: 8328-8333

Campoli C, Shtaya M, Davis SJ, von Korff M (2012). Expression conservation within the circadian clock of a monocot: identification of the barley core-clock genes. BMC Plant Biol. 12:97

Shin J, Heidrich K, Sanchez-Villarreal A, Parker JE, Davis SJ (2012). TIME FOR COFFEE represses accumulation of the MYC2 transcription factor to provide time-of-day regulation of jasmonate signaling in Arabidopsis. The Plant Cell 24 (6): 2470-82

Herrero E & Davis SJ (2012). Time for a nuclear meeting: protein trafficking and chromatin dynamics intersect in the plant circadian system.
Mol Plant. 5(3):554-65

Undurraga SF, Press MO, Legendre M, Bujdoso N, Bale J, Wang H, Davis SJ, Verstrepen KJ, Queitsch C (2012). Background-dependent effects of polyglutamine variation in the Arabidopsis thaliana gene ELF3. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19363-7

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